In-Vitro Cell Response to Strontium/Magnesium-Doped Calcium Phosphate Nanoparticles

Calcium phosphate nanoparticles are highly biocompatible and biodegradable in bone regeneration. On the other hand, strontium magnesium enhance formation of bone. The substitution calcium by is an efficient way to improve biological properties phosphate-based biomaterials. Strontium-doped magnesium-doped with degrees cation 5, 10, 15, 20 mol% respect were prepared precipitation, followed surface functionalization polyethyleneimine (PEI, cationic) or carboxymethylcellulose (CMC, anionic). characterized dynamic light scattering (DLS), zeta potential measurement, scanning electron microscopy (SEM), atomic absorption spectrometry (AAS), energy dispersive X-ray analysis (EDX), powder diffraction (XRD). particles approximately spherical (diameter 40–70 nm). addition considerably decreased internal crystallinity, i.e., doped almost amorphous. cell-biological effects assessed on three different cell lines, HeLa cells, MG63 MC3T3 cells. Cell viability tests (MTT) showed a low cytotoxicity, alkaline phosphatase (ALP) activity was strongly increased, taken up well lines.